TIGSS: Molecular Genomics Core

Genomics Core houses various instruments and lab equipment to support genomics-based research and is located in Reynolds Medical Building 446A and VMR Addition 266. Training and experimental support is available.

Equipment that can be reserved for self-use after appropriate training:

Maxwell 16 (Promega): The Maxwell 16 Integrated System combines compact instrumentation, optimized automated methods, prefilled reagent cartridges, service and support to save time.
Qubit Fluorometer (Life Technologies): Easy to use fluorometer for a variety of DNA, RNA and protein assays.
CFX96 Touch Real-Time PCR Detection System (Bio-Rad): 96 well real time PCR system with six-channel detector with quick set up runs. Monitors amplification traces in real time.
Tape Station (Agilent): The Bio-Plex Pro Wash Station eliminates manual wash steps from Bio-Plex Assays, making them as easy as ELISAs.
Bio-Plex Pro Wash Station (Bio-Rad): The Bio-Plex Pro Wash Station eliminates manual wash steps from Bio-Plex Assays, making them as easy as ELISAs.
GentleMACS Octo Dissociator (Miltenyi): Automated tissue processing to dissociate target tissue into single cell suspensions.
Cytation 5 (BioTek): Cell imaging multi-mode microplate reader that combines automated digital microscopy and conventional microplate detection.
MinION (Oxford Nanopore): Oxford Nanopore DNA sequencer for real time, long-read, and low cost sequencing.
10X Chromium: Chromium Single Cell 3’ performs deep profiling of complex cell populations with high-throughput digital gene expression on a cell-by-cell basis.
Countless Cell Counter (Life Technologies): Generates automated cell and viability counts in as little as 30 seconds.
epMotion 5075 Fluidi Handling Robot (Eppendorf): Easy to program liquid handling robot that can be used for any molecular application that can be automated including NGS library generation, PCR setup, and nucleic acid extraction.
Bio-Plex 200 (Bio-Rad): A suspension array system that supports analysis of protein and nucleic acids in a multiplex solution detection up to 100 biomolecules in a single sample.
High Performance Computing: The TIGGS High Performance Compute Cluster is tailored for bioinformatics and computational Biology applications, sequence assembly, alignment and analysis and many more.
Digital PCR System for absolute quantification of target DNA or RNA molecules using EvaGreen and probe-based Droplet Digital PCR.
iScan (Illumina): Scanner and associated equipment that supports the Illumina’s broad portfolio of genetic analysis assays for genotyping, CNV analysis, DNA methylation, and gene expression profiling.
MiSeq (Illumina): Next-gen sequencer for small sequencing projects requiring longer read length. Ideal for bacterial genomes.
NextSeq 500 (Illumina): Next-gen sequencer for small to mid-scale sequencing projects of genomes, exomes, or transcriptomes.
C1 Single-Cell System (Fluidigm): The C1 Single Cell Auto Prep system employs an innovative microfluidic technology to rapidly isolate and prepare individual cells for genome and transcriptome analysis.
Biomark HD (Fluidigm): Integrated fluidic circuits (IFCs) for automating real-time or end-point read PCR reactions in nanoliter volumes.