IBT Flow Cytometry and Cell Sorting Core-Houston, TX
The IBT Flow Cytometry and Cell Sorting Facility (FCCSF) provides diverse, technically sophisticated flow cytometry and cell sorting services, as well as the scientific expertise necessary to integrate this technology into research projects (Dr. Moczygemba).
Equipment:
BD Biosciences FACSFusion Cell sorter:
- uses electrostatic droplet-based, high speed technology for isolating any combination of fluorescently labeled cell markers
- equipped with 4 lasers (405 nm, 488 nm, 561 nm, and 640 nm) and 15 filters for experiments requiring up to 15 multicolor analysis
- variety of nozzle sizes/pressure configurations for sorting all types of cells including fragile ones
- can sort up to 50,000 events/second in temperature-controlled sample and collection chamber
- certified to perform BSL2 level sorting of single cells into 96 well plates, as well as bulk sorting into two or four tubes
BD Biosciences LSRII analytical flow cytometer:
- equipped with 4 lasers (405 nm, 488 nm, 561 nm, and 640 nm) and 13 filters suitable for experiments requiring up to 13 color analysis plus forward and side scatter data acquisition
- users have access to FlowJo data analysis software to customize their data analysis
Intellicyte iQue Screener Plus
- will provide access to high throughput flow cytometry analysis for drug discovery research
- will support plate-based sampling systems (96- or 384-well plates) that can be integrated with plate-handling robotics and automated cell culture and liquid-handling peripherals
- when fully automated, flow cytometry workstations have a maximal throughput capacity of up to 72 separate 384-well plates per day, this is a 50-100 fold improvement over the throughput of conventional flow cytometers
Flow Cytometry Analyses Commonly Performed:
BD Biosciences FACSFusion Cell sorter:
- cell sorting from cell lines, primary cells and disaggregated tissues
- immunophenotyping, cellular cytotoxicity assays, apoptosis detection, kinetic studies, receptor endocytosis, cell cycle analysis, bacterial analysis, and others as needed.
