Image Analysis Laboratory-Veterinary Medicine & Biomedical Sciences

The mission of the Image Analysis Laboratory is to provide advanced imaging technologies and image analysis tools for a broad range of life sciences research. This includes vital imaging of cellular processes within cells, tissue explants, and organoids at BSL-2 biosafety level, as well as a variety of support services for transmission electron microscopy of biological samples. Support services include individual and group instrument training, workshops, assistance with sample preparation, BSL-2 cell culture facilities, and formal instruction in a graduate lecture/lab course, Optical Microscopy and Live Cell Imaging.

Equipment:

ARIVIS workstation:

• High-performance interactive 3D / 4D rendering on standard PCs and laptops with 3D Graphics Support
• Intuitive tools for stitching and alignment to create large multi-dimensional image stacks
• Powerful Analysis Pipeline for 3D /4D image analysis (cell segmentation, tracking, annotation, quantitative measurement, and statistics, etc)
• Easy design and export of 3D / 4D High-resolution Movies
• Seamless integration of custom workflows via Matlab API and Python scripting
• Data sharing for collaboration

Axio Imager.M2 Motorized Upright Microscope:

• Fluorescence LED Illumination – DAPI, GFP, Cy3, Cy5
• Axiocam 506 color camera and Axiocam HRm(opens in a new tab)
• Automated Multichannel, Z-Stacks, Motorized X-Y Stage for Automated Large Region Tiling
• Objectives: 1.25x, 5x, 10x, 20x, 40x, and 63x with phase and DIC optics
• Apotome.2 Optical Sectioning Slider
• ZEN Blue version 2.3 Software

Zeiss Cell Discoverer 7:

• Laser lines at 458, 477, 488, 514, 543 and 633 nm
• Petri dishes, chamber slides, multiwell plates, plastic or glass, thin or thick vessel bottoms, low skirt or high skirt plates.
• Definite focus, Incubation (Temperature & CO2) and motorized stage
• Zen Blue software complemented with Arivis software

Zeiss ELYRA S.1 Superresolution Microscope:

• Zeiss Axio Observer Z1 Microscope fully motorized
• Laser Lines: 405nm, 488nm, 561 and 643 nm
• Incubator for temperature and CO₂ control
• 3 and 5 grid rotation
• Zen software
• Workstation for Super resolution with 3D Visart, FRET, FRAP and Physiology modules
• Objectives: Plan-Apo 10X/0.45, Plan-Apo 63X/1.4oil, Plan-Apo 100X/1.4oil

Zeiss LSM 780 NLO Multiphoton Microscope:

• 34 Ch spectral GaAsP detection multiphoton microscope
• Laser lines at 458, 477, 488, 514, 543, and 633 nm
• Coherent Chameleon Ultra Ti: Saphire (720-950nm) pulsed laser
• Definite focus, Incubation (Temperature & CO2), and motorized stage
• Zen software FRET, FRAP, Physiology, and 3D VisArt modules
• ISS two-channel FCS and Fast FLIM
• Objectives: Plan-Apo 10X/0.45, Plan-Apo 20X/0.8, Plan-Apo 40X/1.4oil, C-Apo 40x/1.2water, Plan-Apo 63X/1.4oil
• Airy Scan detector with detection area consisting of 32 single detector elements, each of which acts like a very small pinhole.
• 1.5x better resolution than any classic confocal instruments.

Zeiss TIRF3:

• Zeiss Axio Observer Z1 Microscope fully motorized
• Two cameras: High resolution AxioCam MRm and Roper S/W PVCAM
• Laser Lines: 405nm, 488nm, 514 nm, 561 nm
• Incubator for temperature and CO₂ control
• AxioVision 4 Software

Zeiss 510 META Confocal Microscope:

• Zeiss Axiovert 200 MOT microscope
• Laser lines at 458, 477, 488, 514, 543, and 633 nm
• Z-stack collection, spectral emission profiling, and separation
• Multi-time, Physiology, FRET, FRAP software
• Two confocal channels, one spectral detection channel (META), two channels non-descanned detection, and one transmitted light channel
• Objectives: Plan-Neofluar 10x/0.3 NA, Plan-Apochromat 20x / 0.8 NA,Plan-Neofluar 40x / 0.85 NA, , Plan-Neofluar 40x / 1.30 NA Oil, Plan Apochromat 63x / 1.4 NA Oil, C-Apochromat 40x / 1.2 NA Water

Zeiss Stallion Digital Imaging Workstation:

• Xenon fluorescent light source, 300 W with rapid switching (<2 msec) between excitation
  wavelengths
• Synchronization hardware (TTL-based)
• Shutter for transmitted light (25 mm)
• Incubator with CO2 and temperature Control
• 2 x CoolSnap HQ Camera with external ventilation
• Stallion software
• Ratio/FRET Software Module
• Antivibration table

Zeiss Digital Imaging Workstation:

• Zeiss Axioplan Microscope with motorized Z-stage and system components for brightfield, darkfield, phase contrast, DIC, and fluorescence
• Zeiss Axiocam HRc color camera with up to 13-megapixel resolution (4140 x 3096) in each color channel

FEI Transmission Electron Microscope:

• High-resolution TEM with top entry stage
• High-Voltage range: 40 to 100kV in steps of 10kV
• Intensity zoom: allows for constant screen brightness at different magnifications
• Intensity limit: prevents electron beam intensity overload on sample
• Magnification: 25 – 200000 x
• Automatic saving of full exposure sequence
• Integrated Dual Pentium PC with Windows operating system

Veritas Microsdissection System:

• UV Laser Cutting and LCM :
  • UV Laser Cutting ideal for non-soft tissues and capturing large numbers of cells
  • LCM (IR Laser) ideal for a single cell or a small number of cells
• Stage, optical movement, cameras, filters, and objectives are completely computer and software controlled
• Microdissection process is documented and archivable
• Unlimited slide processing in batch mode.
• High-sensitivity, variable integration time, color CCD video camera
• Inverted microscope with 4x, 10x, 20x, and 40x objectives

ImageXpress Pico & BioTek Cytation 7:

• Different Sample Holders: Plates, Slides
• Read Method: Live preview imaging with digital confocal, Kinetic, well-area scanning
• Luminescence wavelength range: 300 – 700nm
• Fluorescence wavelength selection: Five channels with images, spectral scanning without imaging
• Absorbance: 200 to 999 nm, tunable in 1 nm increments, available for well scanning
• Temperature control up to 50° C
• Uses CellReporterExpress Software with Windows 10, Gen 5 ver 3.09

Cryostar NX70 Cryostat:

• LCD touch-screen and joystick controls
• User-adjustable LED lighting
• Motorized and manual sectioning
• Vacutome for generating wrinkle-free sections
• Integrated height adjustments for users
• Rapid response temperature control
• Novel knife carrier feed with better sectioning quality
• Optional Cold disinfection for complete surface disinfection